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KMID : 0368419800230020049
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Journal of Plant Biology
1980 Volume.23 No. 2 p.49 ~ p.54
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The Isolation and Fusion of Pea and Barley Mesophyll Protoplasts
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Lee, Kwang-Woong/李光雄
Cho, Sung Ho/Cha, Hyeon Cheol/曺聖昊/車賢哲
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Abstract
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The optimal conditions for the protoplast isolation from the leaves of pea (Pisum sativum L. cv. Sparkle) and barley (Hordeum vulgare L. cv. Baecdong) were determined in order to achieve a somatic hybridization between teo species.
It was revealed that the use of 0.5M sorbitol as an osmoticum was appropriate for pea. The yield of intact protoplasts was the highest (40%) when pea leaves were incubated in the enzyme solution for 4 hours.
In case of barley, the optimal concentrations of cellulase, pectinase and mannitol as the enzyme solution were 2%, 1% and 0.35M, respectively. And the yield of barley protoplasts was the highest (87%) when leaves were incubated in this enzyme solution for 3.5 hours.
A fusion of protoplasts from pea and barley was induced by PEG treatment enriched with calcium salts within 60 minutes.
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